TB-500 Research Monograph

Origin and parent protein

Thymosin Beta-4 (Tβ4) is a naturally occurring 43-amino-acid actin-sequestering protein originally isolated from thymus tissue. TB-500 corresponds to a defined fragment of this parent protein and was developed as a synthetic research tool for actin-binding pharmacology research.

Sequence and structure

TB-500's sequence is Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln, with N-terminal acetylation. Molecular weight is 2888.2 g/mol. The compound is supplied for research as a lyophilised powder, sealed under nitrogen.

Primary research literature

TB-500 and the parent Tβ4 protein have been studied across cardiac repair models, dermal wound-repair models, angiogenesis research, and inflammatory recovery contexts. Published work includes the Smart et al. characterisation of epicardial neovascularization and the broader Goldstein laboratory body of work on thymosin biology.

Mechanism research focus

Published mechanism literature on Tβ4 and TB-500 centres on actin sequestration, endothelial cell migration, and the contribution of thymosin biology to tissue-repair signalling networks. The 4-amino-acid LKKTETQ motif within the TB-500 sequence has been a particular focus of structure-activity research.

Storage, reconstitution, and handling

Lyophilised TB-500 is stable for months at −20°C in its sealed vial. After reconstitution, working solutions should be stored at 2–8°C and used within 7 days. Avoid repeated freeze-thaw cycles.

Quality and verification

VESPER TB-500 is supplied at ≥99.5% HPLC purity, with sequence identity confirmed by mass spectrometry on every batch. Batch-specific certificates of analysis are included with each vial.

Frequently asked