METABOLIC MONOGRAPH

Semaglutide Research Monograph

Long-acting GLP-1 receptor agonist — incretin-research peptide

Semaglutide is a synthetic 31-amino-acid peptide and a long-acting GLP-1 receptor agonist. It is the most extensively published GLP-1 mono-agonist in current research literature, with foundational pharmacology dating to 2012.

Receptor selectivity

Semaglutide engages the GLP-1 receptor (GLP-1R) with high selectivity. It does not engage the GIP or glucagon receptors, distinguishing it from dual agonists like tirzepatide and triple agonists like retatrutide.

Structure and half-life modification

The 31-amino-acid sequence carries a γGlu-C18 fatty-diacid side chain that drives reversible albumin binding and extends plasma half-life into the once-weekly dosing range. Aminoisobutyric acid substitutions confer DPP-IV resistance.

Primary research literature

Semaglutide's pharmacology research dates to 2012, with the SUSTAIN-, STEP-, and PIONEER-program studies generating an extensive body of literature on glycemic regulation, body-weight regulation, and cardiovascular outcomes from 2017 onward.

Storage, reconstitution, and handling

Lyophilised semaglutide is stable at −20°C in its sealed vial. After reconstitution, working solutions should be stored at 2–8°C and used within 14 days. Avoid repeated freeze-thaw cycles.

Comparison with related research peptides

Semaglutide is most often compared with tirzepatide (dual GLP-1/GIP) and retatrutide (triple GLP-1/GIP/glucagon). See the VESPER /compare/tirzepatide-vs-semaglutide and /compare/semaglutide-vs-retatrutide pages for direct comparisons.

Quality and verification

VESPER semaglutide is supplied at ≥99.5% HPLC purity, with sequence identity confirmed by mass spectrometry on every batch.

Sequence

H-Aib-EGTFTSDVSSYLEGQAAKEFIAWLVRGRG-OH (γGlu-C18 fatty diacid side chain)

Molecular weight

4113.6 g/mol

CAS

910463-68-2

Purity

≥99.5%

Storage

−20°C, protected from light

Vial format

5mg / vial

Frequently asked

Is semaglutide selective for GLP-1?

Yes — semaglutide is a high-selectivity GLP-1 mono-agonist. It does not engage GIP or glucagon receptors.

What is the half-life modification on semaglutide?

A γ-glutamic acid linker plus a C18 fatty-diacid side chain promotes reversible albumin binding and extends plasma half-life.

What is the molecular weight of semaglutide?

Approximately 4113.6 g/mol for the 31-amino-acid peptide with C18 fatty-diacid modification.

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⚠ For research use only. Not for human consumption.